TNF and neoplasm: Previous studies have shown that immune cells infiltrating tumor tissue are highly correlated with tumor progression, so they have become the focus of cancer research in recent years (12).Our KEGG and GO analysis results showed that the Th1 and Th2 cell differentiation, TNF signaling pathway, Cytokine-cytokine receptor interaction, PD-L1 expression and PD-1 checkpoint pathway in cancer, TGF-beta signaling pathway were enriched in the low-score group, which suggest that cuproptosis may be closely related to TIME.