Lymph node invasion exhibited a striking difference, with high S100A4 expression found in 65.38% (n=34) of affected patients, while only 8.70% (n=4) of those without lymph node invasion showed high levels (P < 0.0001). Tumor grade analysis indicated a trend towards higher S100A4 expression with increasing grade, although this was not statistically significant (P = 0.1278). This evidence concerns the gene S100A4 and neoplasm.