In contrast, AMV and WCMV co-infection in N. benthamiana had an increasing trend of Ci, Tr, and Gs contents with the increase in time, whereas at the same time, the Ci, Tr, and Gs contents of AMV and WCMV co-infection were significantly higher than those of AMV and WCMV single infection, or CK. This evidence concerns the gene NDUFB6 and infection.