An oncolytic adenovirus against hepatocellular carcinoma (HCC) was created using such a circuit, which included the activator Gal4VP16, two mutually inhibiting repressors LacI and tetR:Krab, a tumor-specific α-fetoprotein promoter, and sequences for binding tumor-specific miR-21 and normal hepatocyte-specific miR-199- 3p and miRNA-142. This evidence concerns the gene TFPI and neoplasm.