Our results demonstrate that recombinant MVA-expressing engineered CTH522 antigens can induce Th1-biased CD4+ T cells and multifunctional CD8+ T cells in this mouse model, which allows us to investigate the role of MHC-dependent T cells in clearing infection and influencing the immunopathology induced by chlamydial infection. Here, CD8A is linked to chlamydia trachomatis infectious disease.