CD80 and infection: Both MVA-spCTH522 and MVA-CTH522:B7 were able to express the respective recombinant antigens at the in silico predicted sizes, approximately 57.4 kDa for spCTH522 and 63.9 kDa for CTH522:B7, as demonstrated by Western blot analysis using cell extracts taken from human HeLa cells at different time points post-infection with either mock or MVA-spCTH522, MVA-CTH522:B7, or MVA-WT (Figure 1C).