ASCL4 and neoplasm: mRNA levels of Ascl4 were not significantly different between groups, although there was a pattern of increased expression in tumours from C/C and HF/C animals (Figure 8e), while levels of Cpt1a, Acad11 and Srebp1c were all significantly decreased in C/C and HF/C tumours relative to non-tumour tissues (Figure 8f–h).