To determine the effect of dietary manipulation on markers of glycolysis and the PPP, mRNA levels of the rate-limiting enzymes Hexokinase 2 (Hk2), Phosphofructokinase, liver type (Pfkl), Pyruvate kinase M2 (Pkm2) and Glucose-6-phosphate dehydrogenase (G6pdx), were assessed in non-tumour livers from male offspring that did not develop HCC. This evidence concerns the gene PFKL and hepatocellular carcinoma.