In our study, SFRP5 expression was lowest in stage Dukes’ D. Our immunohistochemistry and RNA sequencing data results align with Kirana et al., where analysis of an independent tissue cohort from The Cancer Genome Atlas database on 637 patients revealed significantly lower SFRP5 RNA expression in CRC tumor tissue compared with adjacent normal mucosa. Here, SFRP5 is linked to neoplasm.