To estimate the effect of the migraine-linked genetic variant Kv6.4-L360P, we generated the mutation in Kv6.4-cDNA and in vitro transcribed them before expressing them in Xenopus oocytes, which allowed us to have proper control of the relative expression level of Kv2.1 and Kv6.4 constructs. The gene discussed is KCNB1; the disease is migraine disorder.