The analysis indicated that Wdr5 is positively correlated with PD-1, PD-L1, and Spp1 expression in both human colon cancer (Supplementary Figure S2A–C) and human breast cancer (Supplementary Figure S2D–F) in myeloid cells, epithelial cells, and T cells. The gene discussed is SPP1; the disease is malignant colon neoplasm.