The study utilized the Berkeley (BERK) mouse model of Sickle Cell Disease (SCD) which has specific genetic modifications involving human and murine globins. For certain experiments, wild-type (WT) mice and PAR-1 and PAR-2 deficient mice were used. Additionally, bone marrow (BM) transplantation was conducted where irradiated PAR-1 and PAR-2 mice received bone marrow cells from either the BERK or WT control mice. Here, F2RL1 is linked to sickle cell disease.