In addition, ISO led to an increase in mRNA level of classical-cardiac-hypertrophy- and stress-related genes in the left ventricle, particularly under Ppp2r5d-knockdown conditions, including atrial natriuretic peptide (Nppa), brain natriuretic peptide (Nppb), and myosin heavy chain (Myh7) (Figure 3F). Here, NPPA is linked to cardiac hypertrophy.