In this study, we aimed to determine in the effects of Lactobacillus plantarum B7 (L. plantarum B7) and the combination of Lactobacillus rhamnosus L34 (L. rhamnosus L34) and Lactobacillus paracasei B13 (L. paracasei B13) on liver histology, hepatic fat content, hepatic expression of TLR4 and CD14, serum TNF-α levels, and fecal microbiota in a rat model of NASH. This evidence concerns the gene TNF and metabolic dysfunction-associated steatohepatitis.