Due to the chronic neuroinflammatory nature of AD and its connection to abnormal activation of glial cells, we proceeded to conduct immunofluorescence staining using antibodies targeting specific markers for microglia (ionized calcium-binding adaptor molecule 1 (IBA1) and astrocytes (glial fibrillary acidic protein (GFAP)) (Figure 6A, Figure S1). The gene discussed is AIF1; the disease is Alzheimer disease.