A similar EMT hybrid state can be confirmed by ICC using overexpressing GRHL2 cells in an OE pool, and MCF7 and T47D cells transiently transfected with 1 μg GRHL2-GFP plasmid for 48 h (see Supplementary Figure S4C–E). This evidence concerns the gene GRHL2 and intrahepatic cholangiocarcinoma.