Firstly, eight genes [Acetyl-CoA carboxylase alpha (ACACA), Angiopoietin like 4 (ANGPTL4), BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), Fibrillin 2 (FBN2), Menin 1 (MEN1), Matrix metallopeptidase 14 (MMP14), Tumor protein 73 (TP73), and WW domain-containing transcription regulator protein 1 (WWTR1)] showed a downregulation in lung cancer tissue, indicating a possible “second hit” in tumor suppressor genes responsible for gene inactivation (Figure 3A). This evidence concerns the gene BUB1B and lung cancer.