Thus, in diagnosing PD-L1 that exhibits focal expression in tumor tissues [21,22,23,24,25,26,27,28], ex vivo analysis of NSCLC samples decreased the number of false negatives in the immunohistochemical examination, and so this analysis may be a method of choice in assessing the status of this parameter for cancer cells, especially those with low PD-L1 TPS, and treatment decision making. This evidence concerns the gene CD274 and cancer.