Since PTEN regulates the PI3K/AKT signaling pathway, the effect of miR-204 overexpression was also assessed on this pathway and showed that miR-204 overexpression inhibits the expression of p-AKT and p-PI3K significantly in MCF7 [36] and MDA-MB-231 [23] BC cells. The gene discussed is AKT1; the disease is breast cancer.