In the Western blot and immunofluorescence analyses, the expression levels of pyroptosis-related factors, such as NF-κB, NLRP3, GSDMD, ASC, Caspase-1, and IL-1β, were increased significantly after cerebral ischemia-reperfusion injury [45,53,54]. Here, NFKB1 is linked to Cerebral ischemia.