Similarly, another study reported that Brazilin (isolated from the traditional herbal medicine Caesalpinia sappan L.)suppressed TLR2, TNF-α, IL-1β, and IL-6 levels by inhibiting the TLR2/NF-κB/MAPK signaling pathways in mammary gland tissues, thus preventing S. aureus-induced mastitis [75]. This evidence concerns the gene IL1B and mastitis.