Firstly, with the aim of searching for the differences between the most pathologic intestinal HSCR distal sections (called aganglionic due to the uncompleted innervation, segments closer to the rectum) and the healthier HSCR proximal sections (ganglionic, segments closer to the stomach), we have examined the expression of genes that participate in or are related to the NF-κB pathway, together with neuronal markers (Figure 1a,b) in a cohort of 27 HSCR patients by RT-qPCR. The gene discussed is NFKB1; the disease is Hirschsprung disease.