In this work, 8-shogaol application resulted in significant inhibition of TNF-α-, IL-1β-, and IL-17-mediated inflammation and migration of fibroblast-like synoviocytes derived from rheumatoid arthritis patients (applied at 5 μM and 10 μM) as well as in 3D synovial culture system. This evidence concerns the gene IL1B and rheumatoid arthritis.