SIRT1 and obesity due to melanocortin 4 receptor deficiency: Similarly, zerumbone, a ginger sesquiterpene (applied as 0.01% or 0.025% of the diet for 8 weeks), counteracted the obesity induced by high-fat diet in C57BL/6N mice through activation of AMPK and sirtuins (SIRT1) [34] and 6-gingerol (20 μM, for 8–9 days) promoted browning (favorable metabolic phenotype) in mouse 3T3-L1 adipocytes with the upregulation of SIRT1 and p-AMPK/AMPK together with several other biomarkers [35], as well as it exhibited hypoglycemic effect in high-fat diet-fed rats that was associated with increased phosphorylated (activated) AMPK-α1 and sirtuin-6 [67].