Since neutrophil dynamics might provide essential information for disease progression and IVFC can monitor neutrophil dynamics non-invasively, here, we describe a method that adapts an intravital microscope to an IVFC and uses it to monitor LysM-EGFP-labeled circulating myeloid cells (mainly neutrophils) in a TNFα-induced peritonitis mouse model [20]. Here, TNF is linked to peritonitis.