Immunofluorescence assays identified EOC ascites spheroids as multicellular structures containing ovarian tumor cells (PAX8+Ki67+) and numerous lymphocytes, such as TAMs (CD68+CD206+, CD68+CD86+), T cells (CD8+), DCs (CD11c+) and NK cells (CD56dim, CD56bright) (Supplementary Fig. S1E). This evidence concerns the gene CD86 and ovarian neoplasm.