NR4A1 and neoplasm: In triple-negative breast cancer, combining the assessment of TLSs with tumor budding (TB) showed a positive correlation with OS and RFS, suggesting that the TLS/TB model may surpass the classical tumor-lymph node metastasis staging system in predicting patient outcomes.421 Moreover, in the breast cancer tumor microenvironment, TLS presence is positively associated with high tumor grade, phenotype, necrosis, extensive in situ components, lympho-vascular invasion, and high levels of TILs, as well as with hormone receptor negativity, HER2 positivity, and c-kit expression.