In melanoma mice, blocking LTβR did not affect HEVs, whereas TNFR1/2−/− mice lacked PNAd+ HEVs.241 However, TNF-α does not appear to induce mature HEVs, and a more mature HEV phenotype still requires LTβR signaling.233,242. The gene discussed is LTBR; the disease is melanoma.