ETHE1 and colorectal carcinoma: We constructed stable ETHE1-overexpressing SW48 and SW480 CRC cell lines using ETHE1-overexpressing lentivirus and knocked down ETHE1 in SW48 and HCT116 CRC cell lines using lentiviruses containing two distinct shRNA sequences targeting ETHE1, which were validated at both the protein and mRNA levels (Supplementary Fig. 1C–F).