To investigate the molecular mechanism of lncRNA HAGLROS/miR-135b-3p regulating the malignant progression of breast cancer, we screened the target gene COL10A1 of miR-135b-3p by database prediction and confirmed the binding potential of miR-135b-3p to COL10A1 by relevant assays. Here, COL10A1 is linked to breast carcinoma.