To further validate the results of the in vitro experiments, we collected breast cancer plasma exosomes and breast cancer tissues for qRT-PCR and IHC experiments, which showed that the expression of lncRNA HAGLROS was higher in breast cancer plasma exosomes than that of plasma exosomes from healthy individuals, and that the expression of CD86 in breast cancer tissues was lower than that in paracancerous tissues, while the expression of CD206 was in the opposite direction (Supplementary Fig. 3A, B). The gene discussed is CD86; the disease is breast carcinoma.