5.High Se diet significantly attenuated chronic TAC-induced pulmonary F4/80+macrophage accumulation and activation. Since we previously showed that pulmonary macrophages, the most populated immune cell subset in lung tissue, were significantly increased after HF development [3,6,14], we further determined pulmonary macrophage subsets by detecting F4/80+ immune cells in CD45+ leukocytes. This evidence concerns the gene PTPRC and hydrops fetalis.