Overexpression of THADA (N = 3, Change in FC50 = -0.48 ± 0.14) and loss of expression of CG9826 (N = 3, Change in FC50 = -0.43 ± 0.35) and GluRIIC (N = 4, Change in FC50 = -0.19 ± 0.40) all resulted in increased FC50, which is associated with reduced fat content and suppression of the obesity phenotype. Here, THADA is linked to obesity due to melanocortin 4 receptor deficiency.