(i) CNE identified Ki-67+CD8+ T cells more enriched in the T cell enriched CN in CLR donors, Ki-67+ Treg cells more enriched in the macrophage enriched CN in DII donors, and ICOS+ Treg cells more enriched in the bulk tumor CN in DII donors (Fig 3B, black boxes in rows 1, 4, and 2), showing that immunosuppressive activity is increased in macrophage enriched and bulk tumor CNs in DII donors, while in CLR donors cytotoxic activity is increased in the T cell enriched CN. Here, ICOS is linked to neoplasm.