Based on the calculated feature importance score (Methods), CRTAC1 and NID1 were highly predictive in the discovery cohort (Fig. 7d), while in the validation cohort, NID1 and C1QTNF1 had the most predictive performance in discriminating compensated and decompensated states of the RV samples of PAH participants (Fig. 7e). The gene discussed is CRTAC1; the disease is pulmonary arterial hypertension.