More specifically, iECSema3a mice and Cdh5-CreERT2 controls (abbreviated as iECwt) were fed an HFD for 10 weeks to induce hepatic steatosis, followed by injections with tamoxifen to efficiently delete the Sema3a allele (Fig. 7a and Extended Data Fig. 8a). Here, CDH5 is linked to fatty liver disease.