To address if elevated Igfbp7 protein in TAC mouse heart is due to increased Igfbp7 in cardiomyocytes, multiplex immunofluorescent staining was used, which showed TAC-induced increase of Igfbp7 protein expression in cardiac myocytes—less in cardiac microvascular endothelial cells and myofibroblasts (Extended Data Fig. 2b,c). This evidence concerns the gene IGFBP7 and persistent truncus arteriosus.