The results obtained in Piezo1 KO mice 2 d after TAC supported this hypothesis: the hallmark of calcineurin activation, an increase in the nuclear to cytoplasmic NFAT ratio, indicating translocation of NFAT to the nucleus due to dephosphorylation by activated calcineurin, was clearly evident in Piezo1 KO mice 2 d after TAC (P < 0.01; Fig. 5a,b) but absent in both sham-operated controls and α-MHC-MCM+/− mice subjected to TAC. Here, PIEZO1 is linked to persistent truncus arteriosus.