Biochemically, the present study showed significantly (p < 0.05) low values for the serum levels of glucose, cholesterol, total protein albumin, urea, estrogen, progesterone, FSH, LH, T4, PGF2α, calcium, iron, and selenium (46 ± 0.5 mg/dL, 77.6 ± 9.2 mg/dL, 4.5 ± 0.2 g/dL, 3.3 ± 0.05 gm/dL, 56 ±.1.1 mg/dL, 69 ± 3.5 pg/mL, 0.5 ± 0.04 ng/mL, 3.7 ± 0.01 mU/mL, 2.7 ± 0.1 mU/mL, 4.7 ± 0.3 ng/mL, 34 ± 1.1 pg/mL, 5.4 ± 0.1 mg/dL, 115 ± 0.5 Ug/dL, and 2.8 ± 0.05 Ug/dL, respectively) in the endometritis group compared with the healthy buffaloes. This evidence concerns the gene CD4 and endometritis.