TP53 and cancer: A subsequent study compared WES data of early-passaged cells to RNA-seq data from later passages of H1 and H9 (also known as WA01 and WA09), two of the most commonly used hPSC lines, and observed that cancer-related mutations are mostly acquired during prolonged culturing, predominantly in TP53 but also less frequently in other genes, including EGFR, CDK12, and PATZ1, genes that are deregulated in human tumors and have significant pathological potential [39].