We investigated the functional role of both integrins present in ectosomes derived from primary and metastatic melanoma cell lines, as well as non-transformed melanocytes, to provide a baseline understanding of ectosome-mediated αvβ3 integrin/TNF-α and/or αvβ5 integrin/VEGF signaling in tumor angiogenesis. The gene discussed is TNF; the disease is melanoma.