While ectosomes derived from metastatic WM266-4 cells stimulated VEGFA gene expression to a greater extent than ectosomes from their primary isogenic counterpart, i.e., WM115 cells, for ectosomes derived from the second pair (WM793 and WM1205), the effect was more prominent for ectosomes from primary melanoma cells. Here, VEGFA is linked to melanoma.