When CCA cell lines were treated with either 1 or 10 μg/mL anti-EGFR mAb and then challenged by HD02 Vδ2 γδ T cells, the anti-EGFR mAb-mediated ADCC was only marginal in the short-term assay system using a time-resolved fluorescence-based assay (Supplementary Figure S4). This evidence concerns the gene EGFR and cholangiocarcinoma.