MSH6 and colorectal carcinoma: SIFT, PROVEAN, and PolyPhen-2, together with two other tools (PhD-SNP (version PhD-SNP2.0.7, accessed on 14 April 2024) and SNPs&GO last version 8.0, accessed on 14 April 2024), were used in a comparative in silico prediction analysis to identify three MSH6 missense mutations (G932Q, F1104Q, and E1234Q) that may contribute to protein dysfunction and CRC development [66].