In 2022, DynaMut, DUET, and mCSM were used to predict the structural effect and the impact on gastric cancer hereditary susceptibility of a VUS (c.728G>A p.R243Q) identified in the MSH2 gene in a Tunisian family suspected of having both hereditary diffuse gastric cancer (HDGC) and LS. Here, MSH2 is linked to Leigh syndrome.