This Cas-CHDC-powered electrochemical RNA-sensing technology chip successfully detects six non-small-cell lung carcinoma-related RNAs (miRNA-17, miRNA-155, TTF-1 mRNA, miRNA-19b, miRNA-210, and EGFR mRNA), with results consistent with those obtained from qRT-PCR. Here, TTF1 is linked to non-small cell lung carcinoma.