Additionally, flow cytometry analysis demonstrated a substantial increase in the infiltration of total macrophages (CD11b+F4/80+), CD80+ macrophages (CD11b+F4/80+CD80+), CD4+ T cells (CD3ε+CD4+), CD8+ T cells (CD3ε+CD8+), total myeloid‐derived suppressor cells (MDSCs) (CD11b+Gr‐1+) and MHC class II+ MDSCs (CD11b+Gr‐1+MHC‐II+) in the tumours treated with Il‐12 minicircle‐loaded RBCEVs compared to the control (Figure 5B–D). This evidence concerns the gene CD80 and neoplasm.