To investigate the effect of anlotinib on tumor cell apoptosis, we examined the expression of apoptosis-related proteins (Akt, Erk, Caspase3, Cleaved Caspase3) within the four treatment groups, and the results showed that compared with the control group, both anlotinib and docetaxel treatments alone led to an increase in apoptosis, with a more pronounced effect in the combination treatment group (Figure 8E). The gene discussed is AKT1; the disease is neoplasm.