AKT1 and neoplasm: To investigate the effect of anlotinib on tumor cell apoptosis, we examined representative proteins related to apoptosis such as Akt, Erk, Caspase3 and Cleaved Caspase3 in tumor tissues of the four treatment groups, and the results showed that both anlotinib and docetaxel treatments alone promoted tumor cell apoptosis, and the combination of the two showed a more significant effect (Figure 8E).