PCK2 and non-small cell lung carcinoma: While silencing of PCK2 did not affect the viability of A549 and H1975 cells cultured in regular medium, it significantly reduced the viability of these cells cultured in medium containing 1 mM glucose, indicating that PCK2-mediated gluconeogenesis is required for the optimal growth of NSCLC cell under glucose-restriction conditions (Figure 3C).