Supplementary Table S5 provides detailed information on the mass spectrometry results. The interaction between ZEB1 and components of the NuRD complex was validated through Western blotting using antibodies against these specific components in two CRC cell lines (Figure 6B). In addition, GST pull-down assays demonstrated a direct interaction between ZEB1 and HDAC1 as well as HDAC2 (Figure 6C; Supplementary Figure S4A). This evidence concerns the gene HDAC2 and colorectal carcinoma.