CDK6 and acute myeloid leukemia: To determine whether TP directly influences hepatocyte susceptibility to senescence, we treated AML-12 cells (a mouse hepatocyte cell line) with palbociclib (a selective inhibitor of the cyclin-dependent kinases CDK4 and CDK6) for 5 days to induce senescence and then supplemented the culture medium with either recombinant TP protein or vehicle for an additional 48 hours (Supplemental Figure 3A).