To determine whether TP directly influences hepatocyte susceptibility to senescence, we treated AML-12 cells (a mouse hepatocyte cell line) with palbociclib (a selective inhibitor of the cyclin-dependent kinases CDK4 and CDK6) for 5 days to induce senescence and then supplemented the culture medium with either recombinant TP protein or vehicle for an additional 48 hours (Supplemental Figure 3A). This evidence concerns the gene CDK4 and acute myeloid leukemia.