PHC3 and neoplasm: It has previously been shownthat PH3 accumulation in tumor cells treated with Aurora A inhibitorsis detectable from as early as 4–6 h with microscopy.32,59 This suggests a sensitivity advantage for techniques that can resolvemitotic cells in asynchronous cell samples, so we next explored flowcytometry for the detection of PH3 and P-T288 changes in Jurkat cellstreated in vitro with varying GI50-multiplesof compound 7 or a vehicle control for 8 h.