To study the specific role of hepatic miR-33 in MASL and its progression to MASH and HCC, we employed the conditional miR-33–knockout murine model (miR-33loxP/loxP) bred with an Albumin-Cre to induce the deletion of miR-33 in hepatocytes (HKO) (50). This evidence concerns the gene MAS1L and metabolic dysfunction-associated steatohepatitis.