We used a workflow combining ST with the immune-based (CD45+) scRNA-Seq approach described above (Figure 2A) to obtain spatial gene expression measurements of D2.mdx (a model of DMD) (55) skeletal muscle (gastrocnemius [GAST]) at a DMD disease stage with seemingly intact regeneration capacity (2 months of age) but still carrying a Gpnmb nonsense mutation (Figure 5A). Here, GPNMB is linked to Duchenne muscular dystrophy.