CD8A and neoplasm: For the analysis of tumor-specific methylation, we used a control sample consisting of methylation data obtained from five normal human T–cells—CD3, CD4_1, CD4_2, CD4_3 and CD8—averaged out by Metilene. A pairwise comparison of the methylation status of 7,628,019 cytosines (covered 5×, averaged out by Metilene) that overlapped in all samples revealed that tumors were severely hypomethylated relative to the controls (Figure 2A, Figures S1 and S3).