CDKN2A and colonic neoplasm: To verify whether shikonin should potentially induce cell senescence in colon cancer cells by downregulating the expression of CDKN2A and CXCL8, we used a SA-β-Gal staining experiment to detect the senescent cells in the colon cancer cells that were treated with shikonin (Min et al., 2007; Hong et al., 2020).