We found that shikonin increased the activity of senescence-associated β-galactosidase (SA-β-Gal) in colon cancer cells after downregulation of CXCL8 and CDKN2A through a senescence-associated β-galactosidase (SA-β-Gal) staining experiment. This evidence concerns the gene CDKN2A and malignant colon neoplasm.