Receiver operating characteristic (ROC) curve analysis indicated an AUC value for NF-L of 0.99 ± 0.006 (95% CI 0.98–1.000, P = 0.000) for amyotrophic lateral sclerosis, with the optimal cut-off at 1513 pg/mL determined by the Youden index (0.92; sensitivity of 95.45%; specificity of 97.37%; Fig. 1C). The gene discussed is NEFL; the disease is amyotrophic lateral sclerosis.