Significantly higher NF-L protein levels were detected in amyotrophic lateral sclerosis (4565.63 ± 263.77 pg/mL) when compared to HC (847.86 ± 214.37 pg/mL; P = 0.000) and also when compared to IP (1156.4 ± 356.6 pg/mL; P = 0.000) but not when compared to amyotrophic lateral sclerosis–mimic diseases, such as myelopathy (2900.11 ± 872.20 pg/mL; P = 0.821) and the OMD (3169.75 ± 1096.65 pg/mL; P = 0.63) cases (Fig. 1B). Here, NEFL is linked to amyotrophic lateral sclerosis.