The comparison between the AUC values of both biomarkers using DeLong’s method determined that there are not significant differences between CXCL12 and NF-L (ΔArea 0.02 ± 0.0206, z = −9725, P = 0.33) in diagnosis accuracy, showing similar capabilities to differentiate between HC and amyotrophic lateral sclerosis cases. Here, NEFL is linked to amyotrophic lateral sclerosis.